A theoretical method to determine unstressed off-rate from multiple bond force spectroscopy
نویسندگان
چکیده
منابع مشابه
Hidden multiple bond effects in dynamic force spectroscopy.
In dynamic force spectroscopy, a (bio-)molecular complex is subjected to a steadily increasing force until the chemical bond breaks. Repeating the same experiment many times results in a broad distribution of rupture forces, whose quantitative interpretation represents a formidable theoretical challenge. In this study we address the situation that more than a single molecular bond is involved i...
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Force spectroscopy measurement of rupture forces of bound molecules becomes an important physicochemical tool in characterizing intermolecular interactions. Atomic force microscopy (AFM) measurements are among the most common approaches in implementation of this technique. Kinetic information about the molecular bond under study is usually extracted assuming that the detected rupture force come...
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چکیده: هدف اصلی این مطالعه ی توصیفی تحقیقی در حقیقت تلاشی پساروش-گرا به منظور رسیدن به نتیجه ای منطقی در انتخاب مناسبترین راهکار آموزشی بر گرفته از چارچوب راهبردی مطرح شده توسط والدمر مارتن بوده که به بهترین شکل سازگار و مناسب با سامانه ی آموزشی ایران باشد. از این رو، دو راهکار آموزشی، راهکار ارتباطی و راهکار بازساختی، برای تحقیق و بررسی انتخاب شدند. صریحاً اینکه، در راستای هدف اصلی این پژوهش، ر...
15 صفحه اولA Simple and Practical Spreadsheet-Based Method to Extract Single-Molecule Dissociation Kinetics from Variable Loading-Rate Force Spectroscopy Data.
A simple, practical, and model-free method is presented, by which kinetic data can be extracted from variable loading-rate single molecule dynamic force spectroscopy experiments. The constant sampling rate of digital acquisition facilitates the sorting of multiple force curves into histograms that reflect the collective force-time history of the bonds of interest. Combining the force-time histo...
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Force spectroscopy measurements of the rupture of the molecular bond between biotin and streptavidin often results in a wide distribution of rupture forces. We attribute the long tail of high rupture forces to the nearly simultaneous rupture of more than one molecular bond. To decrease the number of possible bonds, we employed hydrophilic polymeric tethers to attach biotin molecules to the atom...
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ژورنال
عنوان ژورنال: Colloids and Surfaces B: Biointerfaces
سال: 2012
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2012.02.010